CoverageAnalyzer (CAn): A Tool for Inspection of Modification Signatures in RNA Sequencing Profiles

CoverageAnalyzer (CAn): A Tool for Inspection of Modification Signatures in RNA Sequencing Profiles

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Combination of reverse transcription (RT) and deep sequencing has emerged as a powerful instrument for the detection of RNA modifications, rike knife lamella a field that has seen a recent surge in activity because of its importance in gene regulation.Recent studies yielded high-resolution RT signatures of modified ribonucleotides relying on both sequence-dependent mismatch patterns and reverse transcription arrests.Common alignment viewers lack specialized functionality, such as filtering, tailored visualization, image export and differential analysis.Consequently, the community will profit from a platform seamlessly connecting detailed visual inspection of RT signatures and automated screening for modification candidates.CoverageAnalyzer (CAn) was developed in response to the demand for a powerful inspection tool.

It is freely available for all three main operating systems.With SAM file format as standard input, CAn is an intuitive and user-friendly tool that is generally applicable to the large community of biomedical users, starting from simple visualization of RNA sequencing (RNA-Seq) data, up to wahl power grip clippers sophisticated modification analysis with significance-based modification candidate calling.